The first written record of theatre performances taking place in Most dates back to 1681. The turning point in the history of the Most Theatre was the construction of a new theatre building that was pushed through by the German bourgeoisie and which was after some disputes and discussions situated exactly as proposed and designed by the architect Alexandr Graf in 1911. The theatre was officially opened on 30 September 1911.

The development of the theatre was significantly slowed down by the First World War, but the big changes coming after the war and the foundation of Czechoslovakia meant that Czech plays were staged in Most Theatre - the big moment came on 4 April 1919 when the first theatre performance by Czech amateur thespians was staged in the theatre. Unfortunately, most performances were still in German. In 1948 the character of the theatre changed completely, it was now purely Czech and it was renamed the Workers' Theatre; the theathre became very popular.

A new theatre building was built in the 1980s and it was decorated with several unique features, demonstrating the art and skill of the architects and builders involved. For the five and a half years when the new theatre building was being constructed, the theatre ensemble performed in the Máj Cultural Centre and many amazing performances were staged in these temporary premises.

The present auditorium has a capacity of 500 seats.